ABSTRACT
Background: Hemolytic disorders are one of the prime reasons for frequent blood transfusions which involves lots of costs and sufferings to the patient. This study was undertaken to determine the effect of water soluble extract of Aloe vera on rabbit erythrocytes in varying concentrations of NaCl from 0.9% (isotonic) to 0.15% (hypotonic).Methods: Aqueous extract of Aloe vera (AVE) 200mg/kg was orally administered to rabbits in the test group while control group was given 1ml of distilled water (DW). Blood was withdrawn from rabbits, centrifuged and suspension in 1ml of normal saline was made. 20 microliter of red blood cells suspension from both control and test groups was added to normal saline of varying concentrations from 0.9% to 0.15% NaCl which were quantitatively analysed for hemolysis by UV spectrophotometer. Data was analysed by unpaired t test and P <0.05 was considered statistically significant.Results: The difference in percentage of hemolysis in both test and control groups was not statistically significant. Therefore, acute administration of water soluble extract of Aloe vera (200mg/kg) did not have protective effect on rabbit erythrocytes against hypotonic solution of normal saline.Conclusions: Aloe vera might be useful for the treatment of oxidative stress-related human disorders by virtue of its antioxidant activity and may have a role in prevention of hemolysis which needs to be explored by further studies.
ABSTRACT
Background: Pharmacovigilance knowledge and training in post graduate student doctors is key factor for proper implementation of PvPI. Often the dentists and physiotherapists who are also one of the main stakeholders for ADR reporting are neglected for training and knowledge regarding pharmacovigilance. This study was planned to evaluate the knowledge and awareness of pharmacovigilance in post graduate students in tertiary care centre in Indore.Methods: It was a single point cross sectional questionnaire-based study conducted in a tertiary care Institute MGM Medical College and M.Y. Hospital in the state of Madhya Pradesh at Indore. It was conducted among post graduate student doctors from dentistry and physiotherapy. Total of 55 questionnaires were distributed, 50 of them were returned back and were analysed.Results: Overall knowledge level was average. 90% knew about ADR while 80% were aware about PVPI. 10% knew about local AMC at Indore while only 04% knew global centre for Pharmacovigilance is at Sweden Uppsala. 88% thought Med watch as global database for ADR against only 12% knew its Vigibase. 90% thought ADR reporting is necessary. 96% thought it should be included in UG curriculum. 98% had not reported any ADR till date while 84% had not seen an ADR form.Conclusions: Post graduate doctors are the prime candidates to impart the importance of pharmacovigilance. The study strongly suggested that there was a great need to create awareness and impart training among the post graduate doctors to improve the reporting of ADRs.